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Synthetic mRNA

Accelerating discovery by automating mRNA synthesis

A new paradigm in mRNA synthesis

Sequence In. mRNA Out. Overnight.

The BioXp 3250™ enables you to rapidly, accurately and reproducibly build or “write” high-quality synthetic mRNA in a push-button, end-to-end automation system. This consolidated build solution helps you accelerate your overall design-build-test paradigm and advance discovery.

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Accelerate your discovery

  • Synthesize as many as 16 unique, biologically active custom mRNA fragments in a single run (~20 hours)

Enhance your efficiency

  • Automate numerous steps and go from pooled oligos to mRNA.
  • Eliminates manual steps, rigid protocols and time or resource investment from highly skilled FTEs.

Get consistent quality

  • The BioXp™ 3250 uses our Industry standard Gibson Assembly® method and automation workflow to ensure high-quality synthetic mRNA output with every run.

Automate the building of mRNA to remove bottlenecks in your discovery process

Recent advances in mRNA stability, immunogenicity, expression, and delivery have boosted the promise of mRNA as a therapeutic molecule. Rapidly emerging categories of mRNA drugs include vaccines, cell therapies and replacement therapies.

Procuring synthetic mRNA, can often be time consuming and expensive, creating a bottleneck for discovery. The process of building mRNA from sequence is inherently complex and requires numerous steps. This means most researchers are subjected to the long lead times, inconsistency or the tedious workflows of existing mRNA solutions whether in-house or outsourced.

Workflow

"The goal of our research is to develop novel mRNA therapeutics to rare genetic diseases. We are committed to doing so quickly and effectively; however, we are restrained by the large amount of time it takes from design to assessment of the new therapeutic."

Boris Resnick, Scientist II, mRNA Therapy and Research at Ultragenyx.

Ultragenyx
Workflow

Design

  • Use the guided design tools on the BioXp™ design portal to order sequences

BioXp™ 3250

  • Load the custom pooled oligo plates on the BioXp system.

Test

  • After an overnight run, retrieve biologically active mRNAs and the template DNA from the BioXp™ for downstream testing.

BioXp™ mRNA synthesis kit

Now available with Cleancap® mRNA capping technology

Contains all of the Gibson Assembly® reagents necessary to synthesize up to ten micrograms of biologically active capped and tailed synthetic mRNA using de novo synthesized, error-corrected gene fragments (mRNA template) of up to 1.8 kb in length. Optional choices include the selection of the cap method and adding N1-methyl-pseudouridine or pseudouridine nucleosides to the mRNA and unmodified mRNA.

Download mRNA flier

CleanCap logo

Product specifications

Product format: Single-stranded capped and tailed mRNA, and linear double-stranded DNA fragments
Features: 5’ ARCA or CleanCap® AG (3’OMe); poly(A) tail (median 150 As)
Nucleoside modification options:

  • When selecting ARCA: None or Pseudouridine
  • When selecting CleanCap® AG (3’Ome): None or N1-methyl-pseudouridine
Output size: 0.3–1.8 kb per mRNA
Price: Contact us
Order throughput: 1 to 16 samples per run
Configuration: 8 and 16 reactions per run
Yield: 1–10 µg per mRNA (length- and sequence-dependent)
System runtime: ~20 hours
Total turnaround time: 3 to 5 business days from order to shipped kit (International shipping will vary)
Classification: Research use only

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BioXp™ small-scale mRNA synthesis kit

Contact us

The expertise of our scientists, engineers, and supporting teams is always available. Or call us: 858.228.4115

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