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Genome editing

High-throughput design for CRISPR experiments

Genetic modification

CRISPR-powered genome editing has enabled significant improvements in the ability to fine-tune genomes. Originally discovered as an mRNA-based adaptive immune response in E. coli, the CRISPR/Cas9 system contains both guide RNA for sequence-specific targeting and a Cas9 endonuclease that removes foreign DNA and allows integration of synthetic DNA into the host genome. That synthetic DNA is designed to specifically target a region in the host genome and make alterations (e.g. add genes, remove genes, correct mutations).

Genetic modification

Enhance productivity in genome editing workflows

The BioXp™ system enhances productivity during the design phase of the customer’s product development cycle by enabling rapid, automated synthesis of gene fragments, clones, and variant libraries. With the BioXp™ system, scientists can perform rapid, high-throughput gene synthesis and cloning of mRNA expression cassettes, regardless of vector size and complexity. In addition, our Gibson Assembly® RapidAMP™ technology — which permits cell-free amplification of microgram quantities of DNA — means plasmid design no longer has to be tethered to an E. coli cloning system. Our Gibson Assembly® RapidAMP™ technology combines cloning and vector amplification in smaller mini-circle plasmids absent E. coli-based genes, thus improving overall transfection efficiency.

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