Build DNA variant libraries
Synthetic DNA, simplified
BioXp™ libraries kits
BioXp™ libraries kits are available with varied single contiguous amino acid sites, including site-saturation such as NNN, NNK, or any other IUPAC nucleotide code, varied single contiguous alanine sites, or varied, multiple non-contiguous amino acids sites using degenerate bases to optimize protein binding and function.
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.
Scanning libraries
BioXp™ site saturation scanning libraries kits and BioXp™ alanine scanning libraries kits provide a wide variety of scanning libraries with varied single, contiguous amino acid sites, including site-saturation (NNN, NNK) and alanine scanning libraries. Design and build your own custom libraries or let our expert team guide you.
Quickly identify AA sites that are sensitive to effect
Product specifications
Product format: Linear double-stranded DNA
Output: 96-well plate; 32 variants per plate; 1 position per well
Yield: 200 ng–1 µg per position (length-dependent)
Minimum order: 32 variants
System runtime: from 6-7 hours
Total turnaround time: 3-5 days from order to built libraries
Combinatorial libraries
BioXp™ combinatorial libraries kits provide a wide variety of combinatorial libraries with varied, multiple non-contiguous amino acids sites using degenerate bases to optimize protein binding and function. Design and build your own custom libraries or let our expert team guide you.
Identify the optimal combination for the desired effect
Product specifications
Product format: Linear double-stranded DNA
Output: 96-well plate; 32 variants per plate; 1 library per well
Yield: 200 ng–1 μg per position (length-dependent)
System runtime: 6–7 hours
Total turnaround time: 3–5 days from order to built libraries
BioXp™ variant libraries application note
DNA variant libraries are a powerful tool for manipulating protein structure for optimization studies in biologics development, enzyme engineering, and related disciplines. In this application note, we worked with a customer to build high-quality DNA variant libraries with the BioXp™ system, the world’s only full-stack synthetic biology workstation for on-demand DNA assembly and amplification. The customer generated data for two library builds, completing the projects in five days, where using traditional service providers would have taken two weeks to one month — a significant time savings during the design, build, test cycle.
Improvements in target validation rates by automating and streamlining antibody production
Case study: Adopting the BioXp™ system compresses design-build-test cycle times and increases pipeline by 25% for one biopharmaceutical company developing an antibody-based therapeutic
Engineered T cells have been used successfully to treat several leukemias and solid tumors. However, building and screening engineered T cell receptors is time-consuming and labor-intensive. Learn how a biopharmaceutical company transitioned from a traditional method of building expression constructs for chimeric antigen receptors (CAR) to an automated method using the BioXp™ system. The BioXp™ system is the world’s first and only commercially available push-button automated platform for on-demand synthesis of DNA fragments, libraries, and more. The traditional method relied on manual processes for DNA fragment synthesis, cloning, and tracking the reactions. The large number of candidates required for screening made this method prone to human error, cumbersome, and complicated. Adopting the BioXp™ system streamlined the building of expression constructs, eliminating several manual processes from the workflow, and accelerated the identification of lead candidates.
