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DNA amplification

Build and amplify complex DNA constructs quickly

BioXp™ RapidAMP™ cell-free DNA amplification kit

BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes, cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.

Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Cloning without E. coli — get to your results faster

Get to your results faster and in fewer steps with Gibson Assembly® RapidAMP™ isothermal amplification. Assemble and amplify transfection-ready DNA in a simple two-day workflow. Achieve over 10 µg of high-quality, high-fidelity DNA with RapidAMP™ technology, and skip the tedious tasks associated with transformation, cell culture, and E. coli harvest.

RapidAMP Kits

A robust amplification workflow

Begin with a linearized vector, plus up to three linear DNA insert fragments — 300 bp to 1,800 bp each — and assemble via Gibson Assembly® technology to generate a circular cloning product.

A robust amplification workflow chart 01
A robust amplification workflow chart 02

This representative gel shows clean bands, post-restriction digest and before any DNA purification. After cloning and amplification on the bench, or with the BioXp™ system, the final DNA product can be digested to linear form. It is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. RapidAMP™ technology has been used successfully for transfection — lipofection and electroporation — and in vitro transcription. The Gibson Assembly® RapidAMP™ workflow produces clean, high-yield DNA material in only a few hours.

BioXp™ RapidAMP cell-free DNA amplification kit

BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.

Product specifications

Max # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300–1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours

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