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Cell-free DNA Amplification

Simplifying sequence to end-point testing

BioXp® DNA amplification kits

Candidate Sequence to transfection-scale DNA in a week

Telesis Bio’s BioXp DNA amplification kits allow you to amplify DNA constructs to transfection-scale quantities and skip tedious bacterial cultures and erratic service provider delivery times.

Leveraging the power of isothermal DNA amplification technology, the cell-free BioXp DNA amplification kits can be leveraged to redefine your DNA amplification workflow starting from a variety of user inputs.

Whether you are starting from a digital sequence or already have components or constructs on hand, our Select and De novo kit formats can help accelerate your time to transfection results with the push-button, walk-away, end-to-end automation of the BioXp system:

De novo BioXp DNA cloning and amplification kits: Allow users to submit a digital sequence for the generation of linear DNA fragments, clones of interest, and to generate isothermally amplified DNA.

BioXp Select DNA cloning and amplification kits: contain reagents for the generation of clones of interest and isothermal amplification of cloned DNA starting from user-provided linear DNA fragments.

BioXp Select plasmid amplification kits: contain reagents for isothermal amplification of plasmid DNA.

Robust cell-free DNA amplification

DNA amplification workflow

A new paradigm for transfection workflows

A new paradigm for transfection workflows

BioXp® DNA Cloning and amplification kit

Contains Gibson Assembly® and other reagents necessary to clone and amplify 10 micrograms of concatemerized DNA starting from digital insert sequence. The kit supports the use of up to 2 user-supplied linearized vectors per kit.

Product specifications

Product input: Digital insert sequence(s) submitted at ordering containing 40 bp homology overlaps with vector sequence(s) (0.3-1.8 kb length). Linearized, Gibson assembly compatible* vector(s) containing 40 bp homology overlaps with insert sequence(s) (2.7-12 kb length).
Product output: Amplified DNA (branched concatemers), Gibson assembly cloning reaction (target insert cloned into input vectors), dsDNA insert fragments
Minimum yield: 10 μg amplified DNA
Order throughput: up to 22 samples per run
Configuration: BioXp 3250: 14, 22 reactions | BioXp 9600: 14, 22, reactions
System: BioXp 3250 and 9600 systems
System runtime: ~24.5 hours on both BioXp 3250 and 9600 systems
Classification: Research use only

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BioXp® Select DNA cloning and amplification kit

Contains Gibson Assembly® and other reagents necessary to synthesize at least 10ug of amplified DNA from fragments and linearized vectors supplied by the user.

Product specifications

Product input: Mix of insert and vector fragments in T low E. Linear DNA inserts sourced from Telesis Bio DNA fragments, IDT gBlocks™, Twist Biosciences, and Gibson assembly compatible vector with 40bp homology between vector and insert.
Product output: Amplified DNA (branched concatemers), Gibson assembly cloning reaction (user insert cloned into input vector)
Minimum yield: 10 μg amplified DNA
Order throughput: up to 96 samples per run
Configuration: BioXp 3250: 8, 16, 24, reactions | BioXp 9600: 8, 16, 24, 48, 96 reactions
System: BioXp 3250 and 9600 systems
System runtime: BioXp 3250: 10 hours | BioXp 9600: ~ 11 hours
Classification: Research use only

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BioXp® Select plasmid amplification kit

Contains reagents necessary to generate at least 10 micrograms of amplified DNA starting from a sequence verified plasmid supplied by the end user.

Product specifications

Product input: Plasmid supplied by user (sizes: 1.8-37 kb)
Product output: Amplified DNA (branched concatemers)
Minimum yield: 10 μg amplified DNA
Order throughput: up to 24 samples per run on BioXp 3250 system | up to 96 samples on BioXp 9600 system
Configuration: BioXp 3250: 8, 16, 24, reactions per run | BioXp 9600: 8, 16, 24, 48, 96 reactions per run
System: BioXp 3250 and 9600 systems
System runtime: 7 hours
Classification: Research use only

Order

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