Build and amplify complex DNA constructs quickly
BioXp™ RapidAMP™ cell-free DNA amplification kit
BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes, cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.
Cloning without E. coli — get to your results faster
Get to your results faster and in fewer steps with Gibson Assembly® RapidAMP™ isothermal amplification. Assemble and amplify transfection-ready DNA in a simple two-day workflow. Achieve over 10 µg of high-quality, high-fidelity DNA with RapidAMP™ technology, and skip the tedious tasks associated with transformation, cell culture, and E. coli harvest.
A robust amplification workflow
Begin with a linearized vector, plus up to three linear DNA insert fragments — 300 bp to 1,800 bp each — and assemble via Gibson Assembly® technology to generate a circular cloning product.
This representative gel shows clean bands, post-restriction digest and before any DNA purification. After cloning and amplification on the bench, or with the BioXp™ system, the final DNA product can be digested to linear form. It is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. RapidAMP™ technology has been used successfully for transfection — lipofection and electroporation — and in vitro transcription. The Gibson Assembly® RapidAMP™ workflow produces clean, high-yield DNA material in only a few hours.
BioXp™ RapidAMP cell-free DNA amplification kit
BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.
Max # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300–1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours