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VMAX™ X2 CELLS

Robust expression and growth with advantages over E. coli

Vmax™ X2 chemically competent cells cells are derived from one of the fastest growing organisms on earth: the gram-negative, non-pathogenic marine bacterium, Vibrio natriegens. Engineered as a next-generation bacterial expression system, Vmax™ X2 cells retain all the benefits of traditional bacterial protein expression systems — with doubling two times faster than E. coli — and the ability to generate significantly greater amounts of biomass and protein per volume of cells.

More protein in a synthesized workflow

"Using Vmax™ cells in place of conventional E. coli strains allowed us to reduce our media costs in half due to the 2–4X higher expression yields and the ability to induce Vmax™ cells before reaching exponential phase."

Klaus Zangger | University of Graz

2–4x higher protein yield

2–4x higher protein yield

Generate more soluble protein in less time than E. coli for a more efficient expression system

Induce expression early

Induce expression early

Shorten protein expression workflows and have the flexibility to induce expression early

100x lower endotoxin

100x lower endotoxin

Naturally low immunogenicity with lower endotoxin levels than E. coli

Start analyzing your results sooner

Reach protein purification and analysis faster with Vmax™ X2 cells.

Vmax™ X2 Cells

Incorporates into your existing workflow

Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21 (DE3) and have a transformation efficiency rivaling the best alternative protein expression strains. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ X2 cells can be induced over a wide range of timepoints, from an OD600 of 0.1 to over 1.0, without loss of final protein yield, dramatically increasing workflow flexibility.

Vmax™ X2 cells

Product specifications

Strain type: T7 expression strain
Genotype: Vibrio natriegens 14048 dns::LacI-T7-RNAP
Transformation efficiency: 107 CFU/μg DNA

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