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Gibson Assembly® kits

The world’s most trusted method to build seamless high-fidelity DNA

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Simple, robust protocols

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Seamlessly assemble large, complex constructs

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High-fidelity, high-efficiency assemblies

Gibson Assembly® Ultra

Gibson Assembly® Ultra kits

Contains all the reagents necessary to simultaneously assemble as many as 10 DNA fragments into a vector to produce a final product that is several hundred kilobase pairs in length

Gibson Assembly® RapidAMP™

Gibson Assembly® RapidAMP™ kits

Contains all the reagents necessary to simultaneously assemble and clone DNA using Gibson Assembly, and then amplify the resultant product up to ~10 µg DNA

Gibson Assembly® HiFi

Product specifications

Maximum # of fragments assembled: 5
Cloning efficiency: 90% full-length clones
Insert size range: 500–32,000 bp
Total construct size range: 100,000 bp
Reaction time: 60 minutes

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Gibson Assembly® Ultra

Product specifications

Maximum # of fragments assembled: 10
Cloning efficiency: 95% full-length clones
Insert size range: 100–100,000 bp
Total construct size range: 1,000,000 bp
Reaction time: 80 minutes

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Gibson Assembly® RapidAMP™ Ultra kit

Product specifications

Maximum # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300–1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours

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Optimize your workflow with the BioXp™ system

Revolutionizing synthetic biology

Gibson Assembly® reagents are available in a benchtop reagent kit or in automated format, compatible with the BioXp™ 3200 system and BioXp™ 3250 System.

The BioXp™ system enables up to 32 constructs to be built, cloned into any vector of interest (up to 4 vectors per run), and amplified to > 10 µg transfection-ready DNA in a single overnight run, completely eliminating the need to transform into E. coli.

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