Gibson Assembly® kits
The world’s most trusted method to build seamless high-fidelity DNA
Simple, robust protocols
Seamlessly assemble large, complex constructs
High-fidelity, high-efficiency assemblies
Gibson Assembly® Ultra kits
Codex DNA's Gibson Assembly® Ultra reagent kits provide the longest, highest-fidelity builds available.
Gibson Assembly® RapidAMP™ kits
Gibson Assembly® RapidAMP™ kits take it one step further and provide amplified, transfection-ready quantities of > 10 μg of DNA in an endotoxin-free reaction.
Gibson Assembly® HiFi
Product specifications
Maximum # of fragments assembled: 5
Cloning efficiency: 90% full-length clones
Insert size range: 500–32,000 bp
Total construct size range: 100,000 bp
Reaction time: 60 minutes
Gibson Assembly® Ultra
Product specifications
Maximum # of fragments assembled: 15
Cloning efficiency: 95% full-length clones
Insert size range: 100–100,000 bp
Total construct size range: 1,000,000 bp
Reaction time: 80 minutes
Optimize your workflow with the BioXp™ system
Revolutionizing synthetic biology
Gibson Assembly® reagents are available in a benchtop reagent kit or in automated format, compatible with the BioXp™ 3200 system.
The BioXp™ system enables up to 32 constructs to be built, cloned into any vector of interest (up to 4 vectors per run), and amplified to > 10 µg transfection-ready DNA in a single overnight run, completely eliminating the need to transform into E. coli.
Only with the BioXp™ system is it possible to shorten the design-build-test cycle to just two days.
Gibson Assembly® RapidAMP™ Ultra kit
Product specifications
Maximum # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300–1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours