The only next-generation bacterial expression system
Vmax™ X2 cells are derived from one of the fastest growing organisms on earth: the gram-negative, non-pathogenic marine bacterium, Vibrio natriegens.
Vmax™ X2 cells double two times faster than E. coli and generate significantly greater amounts of biomass and protein per volume of cells.
Simply stated, Vmax™ X2 cells result in much larger amounts of soluble protein, much faster.
Join us in saying #GoodbyeEcoli
VMAX™ X2 COMPETENT CELLS
A new host system with advantages over E. coli
Vmax™ X2 competent cells are engineered to retain the benefits of traditional bacterial protein expression systems — low cost, easy to grow, high transformation efficiency, and compatible with plasmids and antibiotics already in widespread use.
With an accelerated doubling time, Vmax™ X2 cells shorten protein expression workflows by one day and generate more soluble protein in less time than E. coli.
Additionally, Vmax™ X2 cells naturally have low immunogenicity with endotoxin levels 100 times lower than E. coli, making them an ideal host system for protein purification.
Incorporates into your existing workflow
Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21 (DE3) and have a transformation efficiency rivaling the best alternative protein expression strains. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ X2 cells can be induced over a wide range of timepoints, from an OD600 of 0.1 to over 1.0, without loss of final protein yield, dramatically increasing workflow flexibility.
Klaus Zangger | University of Graz
Using Vmax™ cells in place of conventional E. coli strains allowed us to reduce our media costs in half due to the 2–4X higher expression yields and the ability to induce Vmax™ cells before reaching exponential phase.
Strain type | T7 Expression Strain
Genotype | Vibrio natriegens 14048 dns::LacI-T7-RNAP
Tube format | Single 50μl rxn per tube
Transformation efficiency | 10^7 CFU/μg DNA