Advances in stem cell therapy afford an alternative therapeutic strategy for treatment of human diseases in clinical therapy. Due to their unique characteristics, stem cells are considered a potential tool to understand and model many critical diseases such as cancer, neurodegeneration, and cardiovascular disease.
Compared with multipotent embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), successfully generated from somatic cells by transfecting four transcription factors (Oct4, Sox2, Klf4, and c-Myc) in fibroblasts, could overcome the limitations of multipotent stem cells that commit to differentiate into only several lineage cells and the ethical concern of ESCs that need the oocytes and embryo destruction.
Further, iPSCs also possess abilities in generating other stem cells and a variety of immune cells for broad clinical requirements. Therefore, utilizing iPSCs in stem cell therapy has great attractions and provides tremendous applications in regenerative medicine.
Advancing regenerative medicine
While human induced pluripotent stem cell (hiPSC) culture has become routine in the lab, pluripotent cell media costs, frequent media changes, and reproducibility of differentiation have remained restrictive, limiting the potential for large-scale projects.
High-throughput solutions on the BioXp™ 3200 system increase turnaround time and provide the flexibility to rapidly iterate beyond what was previously possible particularly in the optimization and formulation of novel hiPSC culture mediums using codon-optimized recombinant proteins.