Originally pioneered by SGI-DNA’s own Dan Gibson, the Gibson Assembly® method is an efficient and robust cloning workflow that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.

SGI-DNA offers several Gibson Assembly® cloning kits for high performance DNA assembly, including the popular HiFi 1-step master mix and the Ultra 2-step master mix for the highest cloning efficiency available and broad fragment size ranges, ideal for complex projects.

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