Originally pioneered by Codex DNA Chief Technology Officer, Dan Gibson, the Gibson Assembly® method is an efficient and robust cloning workflow that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.

Codex DNA offers several Gibson Assembly® cloning kits for high-performance DNA assembly, including the popular 1-step, HiFi master mix, and the 2-step, Ultra master mix for the highest cloning efficiency available and broad fragment size ranges, ideal for complex projects.

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